Mass mortalities associated with viral nervous necrosis in hatchery-reared groupers [Epinephelus coioides and E. akaara] in the people's republic of China
2001
Lin, L. (Zhongshan Univ., Guangzhou (China)) | He, J. | Mori, K. | Nishioka, T. | Wu, J.L. | Weng, S. | Mushiake, K. | Arimoto, M. | Nakai, T.
The viral etiology of mass mortalities of groupers, Epinephelus coioides and E. akaara, cultured in the People's Republic of China was examined. Disease outbreaks occurred in 7 to 45 day-old fish with erratic swimming motion and marked vacuolation was observed in the brain and retina of the affected fish. The piscine nodavirus (the Betanodavirus), the causative agent of viral nervous necrosis (VNN), was detected in the affected tissues by electron microscopy, indirect fluorescent antibody test and reverse transcription-polymerase chain reaction. This paper is the first record of the agent in the People's Republic of China.
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