Characterization of duck microsatellite repeat sequences
2001
Takahashi, H. (National Inst. of Agrobiological Sciences, Tsukuba, Ibaraki (Japan)) | Satoh, M. | Minezawa, M. | Purwadaria, T. | Prasetyo, H.
We cloned duck microsatellite repeat sequences from genomic DNA of an Indonesian native duck to evaluate the genetic relationships among native breeds of duck. A (CA) subn- enriched library was constructed using the method of Takahashi et al. (1996) with modifications. In the library, 8.3% of the clones were CA-positive. The average number of uninterrupted (CA) subn repeats among 22 duck microsatellites was 11.4. Fifteen clones which could be developed as PCR primer pairs to detect (CA) subn repeat length polymorphisms were identified. They should provide a valuable tool for studies related to biodiversity and population structure in duck.
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