Detection of mitogen-induced lymphocyte proliferation by bromodeoxyuridine (BrdU) incorporation in the chicken
2002
Motobu, M. (Japan Science and Technology Corp., Kawaguchi, Saitama) | El-Abasy, M. | Na, K.J. | Hirota, Y.
sup3 H-thymidine (sup3 H-TdR) incorporation assay has been generally used to measure lymphocyte proliferation in the chicken. Disadvantages of this assay are that radioisotope is biological hazard to the person and environment and that it can not measure which subset of lymphocytes proliferates. In this study, bromodeoxyuridine (BrdU) incorporation assay by flow cytometry was compared with sup3 H-TdR incorporation assay. As a result, BrdU incorporation assay showed a strong correlation with sup3 H-TdR incorporation assay, and it could be applied simultaneously to detect BrdU incorporation and expression of cell surface marker antigens. These results suggest that the BrdU incorporation assay by flow cytometry is useful to analyze lymphocyte proliferation in detail.
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