Evaluation of selective plating media for the detection of heat- or freeze-injured Staphylococcus aureus
2002
"Onoue, Y. (Kanagawa-ken. Environmental Center, Yokohama (Japan)) | Shindo, R. | Teranishi, H. | Furukawa, I. | Hasegawa, Y. | Maruyama, T. "
"The efficacy of Baird-Parker (BP) agar, mannitol-salt-egg yolk (MSEY) agar and mannitol salt (MS) agar in detecting Staphylococcus aureus FRI-100 heated at 52degC for 20 min in 100mmol/L potassium phosphate buffer was determined. Brain heart infusion agar with 1% pyruvate (BHIP agar) supported the highest recovery of injured cells and was used as the control medium. Of the three selective media, significantly higher recovery of heat-injured cells was observed on BP agar than MSEY agar, and the poorest recovery was observed on MS agar (p<0.05). Low recovery of unheated cells was obtained for MS compared with other media (p<0.05). A reduction in populations occurred gradually in reagent-grade water stored for 14 days at - 20degC There was no significant difference between BHIP agar and MS agar in the number of freeze-injured cells recovered from 1 to 14 days."
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