In vitro development of bovine nuclear transfer embryos reconstructed with mammary gland epithelial cells at different passages
2003
Kishi, M. (Snow Brand Milk Products Co. Ltd., Sapporo (Japan)) | Itagaki, Y. | Sudo, T. | Takakura, R.
In the present study, the effect of passage of nuclear donor cells on the in vitro development of nuclear transfer (NT) embryos was investigated using colostrum-derived mammary gland epithelial (MGE) cells at different passages (3-30 passages) to find reliable passages for the efficiency of cloning. Development of NT embryos to the blastocyst stage was affected by the number of passages of MGE cells (P< 0.05). Nuclear transfer embryos reconstructed with MGE cells at 3-7 passages showed a significantly higher blastocyst development (31.3-48.5%) than those with the cells at 10-30 pas-sages (2.5-12.5%, P< 0.05). No difference in the proportion of the MGE cells with normal diploid was observed among passage of 3.15 and 30 (P> 0.05). The use of MGE cells at early passages for nuclear donor cells may be advantageous for the production of NT embryos.
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