Development of Biologically Active Compounds from Edible Plant Sources-XI. Analysis of Phytol Content in Several Varieties of Lactuca sativa L. by gas chromatography
2004
Song, M.C. (Kyung Hee University, Suwon, Republic of Korea) | Pai, T.K. (Anyang University, Anyang, Republic of Korea) | Kim, I.H. (Korea Food Institute, Sungnam, Republic of Korea) | Lee, J.H. (Kyung Hee University, Suwon, Republic of Korea) | Bang, M.H. (Kyung Hee University, Suwon, Republic of Korea) | Kim, J.Y. (Kyung Hee University, Suwon, Republic of Korea) | Chung, D.K. (Kyung Hee University, Suwon, Republic of Korea) | Baek, N.I. (Kyung Hee University, Suwon, Republic of Korea), E-mail: [email protected]
An easy, sensitive and rapid method for the analysis of phytol, a strong ACAT (Acyl-CoA: Cholesterol Acyltransferase) inhibitory diterpenoid, was established. The pre-treatment of sample for GC analysis of phytol includes aq. MeOH extraction, partition of the extracts between EtOAc and water, and filtration of organic phase through Supelclean LC-Si SPE tube. The GC analysis of phytol for a variety of Lactuca sativa was carried out on DB-5 capillary column with flame ionization detector (FID). The consistency in phytol contents according to each variety was not shown. Especially, Jeoktoma, as RS-2 type, showed the highest phytol content.
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