Immunological and toxicological studies of Staphylococcin bac188 (Abacteriocins/Bacteriocins-like inhibitory substance) on experimental animals
2004
Saeed, S. | Rasool, S.A. | Ahmad, S.,Karachi Univ. (Pakistan). Dept. of Microbiology)Ali,S.A
Staphylococcin Bac-188, a bacteriocins/ Bacteriocins-like Inhibitory Substance (BLIS) fromStaphylococcus aureus AB-188 was studied for its immunological and toxicological effects onexperimental animals. The antigenicity/ immunogenicity of Staphylococcin Bac 188 wasdetermined by Ouchterlony technique and Enzyme Linked Immunosorbent Assay (ELISA).Accordingly, staphylococcin Bac-188 is antigenically poor (in rabbit immune system) as theantibody titer was not found to be significant in Ouchterlony test. However, someimmunogenicity was witnessed in ELISA, suggesting that low level of antigen-antibody reactionhas occurred. The acute and chronic toxic studies with Bac-188 do not show any toxic effectsin diseases virgin (domestic rabbits) when injected with different regimen of doses (20, 40, 60and 80 gg mL-1). Hematological studies with staphylococcin Bac-188 showed that except formarginal increase in lymphocyte count in the test animal groups, no other significant effectwas observed as compared to the control (rabbits injected with plain sterile saline). Further,different doses of staphylococcin Bac-188 showed no significant biochemical changes in thenormal profile of rabbit blood biochemistry.
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