Association between amylose content and a microsatellite marker across exotic rice germplasm
2003
Bergman, C.J. | Fjellstrom, R.G. | McClung, A.M.
A microsatellite sequence at the waxy gene of rice explains much of the variation in apparent amylose (AA) content of nonwaxy U.S. germplasm. Since the amylose content of breeding lines is typically evaluated across multiple years and locations, this microsatellite shows good promise for marker-aided selection of amylose content. Our study examined the association between the microsatellite and AA using approximately 200 accessions from 53 countries. A previously unreported allele , (CT)10, was found in accessions that had AA in low, intermediate, and high classes. A broader range in AA contents was identified for (CT)20, (CT)18, (CT)16, and (CT)8 alleles than was previously reported. The variance of AA explained by the microsatellite for nonwaxy accessions and known mutants was 68%. The results indicate that this microsatellite marker can be a useful method for predicting the AA content class of a diversity of rice germplasm. However, the relationship of AA content with this microsatellite can be confounded by production environment and analytical methods for determining AA, as well as by other genes and mutations that may occur in the genome.
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Эту запись предоставил International Rice Research Institute