Endophytic fungi from indigenous dicotyledonous plants at Doi Suthep-Pui area, Thailand
1997
Saisamorn Lumyong | Pipob Lumyong | Songumnart Pongsomboon | Hyde, Kevin (Chiang Mai Univ., Chiang Mai (Thailand). Faculty of Science. Dept. of Biology)
Thirty two indigenous dicotyledonous plant seedling species and leaves from a mature tree of Shorea roxburghii collected from Doi Suthep-Pui area were examined for endophytic fungi. Isolation was achieved by a standard triple sterilisation technique, using surface sterilized leaf disc or stem sections plated onto water agar or 2 percent corn meal agar with rose bangal. Avancing regions of mycelium growing from the tissue were transfered to corn meal slants and incubated to promote sporulation. Four hundred fungal isolates were obtained and these were sort into strains. Five to fifteen endophytic species occurred on each plant species, although as many as 50 percent of these are mycelia sterilia. The largest group of endophytes was isolates from the mature tree. Many of the fungi isolated belong in common endophyte genera eg. Phomopsis spp., Fusarium spp., Colletotrichum spp., Phoma spp., and Curvularia spp. Several of the isolates are sterile Xylariaceae (Xylaria spp. and Hypoxylon spp.) and less common species include Nigrospora sp., Alternaria sp., Pestalotiopsis sp., and Penicillium sp. One isolate was an interesting species of Triangularia that may be new to science.
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