Wheat tolerance testing to boron by an in vitro technique and molecular markers-microsatellites
2005
Marjanovic, M.
Wheat tolerance to high boron concentrations was determined by using an in vitro technique of mature embryo culture on modified MS nutrient medium to which boron acid was added in surplus. In preliminary experiment conducted on 7 wheat genotypes in nutrient medium boron acid in concentrations 10, 20, 30, 40 mM was added, while in experiment with 112 wheat genotypes concentrations were 15 and 30 mM. Microsatellite marker Xgwm46 was used for testing, because it was presumed to be in nearness of region on 7B chromosome of wheat which is responsible for boron tolerance. In this experiment a possibility of establishing marker-trait association compairing to results obtained in in vitro testing was examined. Adding of boron acid in nutrient medium caused a reduction in calli growth among all examined genotypes, which significantly discriminated in tolerance to boron high concentrations. 7 different alleles were detected in microsatellite locus (53 genotypes had allele F, 45 genotypes had allele G, 6 genotypes was established to have allele E, 4 genotypes had allele A, 2 genotypes had allele C, 2 had allele B and only one had allele D). Presence of nulli allele was detected at five genotypes. Genotypes with alleles E and C are proposed to be tolerant to high boron concentrations, and they are recommendable like parents in crossing in breeding for high boron concentrations.
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