Somatic embryogenesis in Pinus nigra: embryogenic tissue initiation, maturation and regeneration ability of established cell lines

Salajova, T.. Ustav Genetiky a Biotechnologii Rastlin; Salaj, J.E-mail:nrgrtesa@savba.sk

Somatic embryogenesis in Pinus nigra: embryogenic tissue initiation, maturation and regeneration ability of established cell lines

Salajova, T.(Slovenska Akademia Vied, Nitra (Slovak Republic). Ustav Genetiky a Biotechnologii Rastlin) | Salaj, J.E-mail:nrgrtesa@savba.sk

The effect of plant growth regulators (PGR), 6-benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), alpha-naphthaleneacetic acid (NAA) and sugars (sucrose, maltose, glucose, fructose) on the initiation of somatic embryogenesis of Pinus nigra was investigated. Megagametophytes containing immature zygotic embryos were used as explants. Embryogenic tissue initiation occurred on all the media tested, including PGR-free medium. Relatively high initiation frequencies were obtained on media containing either NAA (9.09%) or 2,4-D (7.14%) alone. Somatic embryos were present as bipolar structures and showed differences in morphological features among cell lines. Plantlet regeneration occurred in cell lines containing bipolar somatic embryos composed of compact meristematic embryo "head" and suspensor organized into bundles.

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