Cell death behind invisible symptoms: early diagnosis of ozone injury
Faoro, F.(Universita di Milano (Italy). Istituto Di Virologia Vegetale)E-mail:[email protected] | Iriti, M.
A simple histo-cytochemical method, combining Evans blue staining to assess cell death and in vivo 3,3'-diaminobenzidine uptake for H2O2 localisation, was used to evaluate ozone damages in leaf tissues of three Phaseolus vulgaris cultivars, Canellino, BLF, Saxa, with different sensitivity to the pollutant. Cannellino was the most sensitive cultivar, showing chlorotic spots 2 h after fumigation with ozone. In BLF, necrotic spots appeared 4 h after fumigation, whereas Saxa remained symptomless up to 10 d of observation. The early appearance of symptoms in Cannellino correlated with H202 accumulation in leaf tissues and consequent extensive cell death. H2O2 accumulation was observed also in BLF, whereas in Saxa leaves, it was found only in the epidermal cells, though the number of dead cells was very similar to BLF, at least up to 24 h after fumigation.
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