In vitro propagation of Cassia angustifolia through leaflet and cotyledon derived calli
2006
Agrawal, V.(University of Delhi (India). Dept. of Botany) E-mail:[email protected] | Sardar, P.R.(University of Delhi (India). Dept. of Botany)
Dark brown compact callus was induced at the cut ends of Cassia angustifolia explants on MS medium augmented with 1 microM N6-benzyladenine (BA) + 1 microM 2,4-dichlorophenoxyacetic acid (2,4-D). Shoots appeared after transfer on a medium supplemented with BA or kinetin. Of the two cytokinins, 5 microM BA was optimum for eliciting morphogenic response in 83.33 and 70.83% cultures with an average of 4.16+/-0.47 and 3.70+/-0.56 shoots in cotyledon and leaflet derived calli, respectively. The addition of 0.5 microM alpha-naphthaleneacetic acid (NAA) to MS + 5 microM BA further elevated the maximum average number of shoots to 12.08+/-1.04 and 5.37+/-0.52 for cotyledon and leaflet calli, respectively. The excised shoots were transferred to a rooting medium containing either indole-3-acetic acid, indole-3-butyric acid (IBA) or NAA. Nearly 95% shoots developed an average of 5.4+/-0.41 roots on half strength MS medium supplemented with 10 microM IBA.
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