Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu
2006
Wang, H.C.(Academia Sinica, Taipei (China). Inst. of Plant and Microbial Biology) | Chen, J.T.(National Univ. of Kaohsiung (China). Inst. of Biotechnology) E-mail:[email protected] | Chang, W.C.(Academia Sinica, Taipei (China). Inst. of Plant and Microbial Biology)
Plant regeneration through somatic embryogenesis of Areca catechu was established using leaf, root and stem segments as explants. Embryogenic callus was induced and maintained on medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 3,6-dichloro-2-methoxybenzoic acid (dicamba) at concentrations 2, 4, 6 and 8 mg/cubic dm in darkness. Somatic embryos were found on primary callus in the presence of 2 and 4 mg/cubic dm dicamba and during subculture on 2-8 mg/cubic dm 2,4-D or 2-4 mg/cubic dm dicamba-containing media. Plantlet conversion from embryos was successfully achieved on growth regulator-free medium. The plants grew well when transplanted to containers in shaded greenhouse.
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