Effects of delipidation and oxygen concentration on in vitro development of porcine embryos

The effects of delipidation and the oxygen (O2) concentration in the atmosphere during culture on in vitro development and H2O2 content were investigated in porcine in vivo fertilized embryos and embryos after in vitro maturation and in vitro fertilization (IVM/IVF embryos). There was no significant difference in the developmental rates to the blastocyst stage between the intact and delipidated IVM/IVF embryos. However, the mean number of cells in blastocysts derived from delipidated IVM/IVF embryos (19.8 +- 0.8 cells) was significantly smaller than that from intact embryos (24.2 +- 1.2 cells). Although there were no significant differences in the developmental rates to the blastocyst stage of intact and delipidated IVM/IVF embryos between, the cultures under 5% O2 and 20% O2, the developmental rate of intact IVM/IVF embryos cultured under 5% O2 (27.1%) was significantly higher than that of the delipidated embryos cultured under 20% O2 (19.3%). On the other hand, there was no difference in the developmental rate to the blastocyst stage between in vivo fertilized embryos cultured under 5% O2 and 20% O2. Hydrogen peroxide (H2O2), one of the reactive oxygen species (ROS), is thought to cause damage to embryos. The H2O2 content per embryo derived from oocytes cultured under 5% O2 (in vivo fertilized, 58.0 +- 2.5 pixels; IVM/IVF, 79.6 +- 3.2 pixels) was significantly lower than that (in vivo fertilized, 100.2 +- 3.8 pixels; IVM/IVF, 103.9 +- 3.2 pixels) under 20% O2. Furthermore, the level of H2O2 in delipidated IVM/IVF embryos (94.7 +- 3.9 pixels) was significantly lower than that in intact embryos (103.9 +- 3.2 pixels) cultured under 20% O2. The present results indicate that the delipidation of porcine IVM/IVF embryos and reduction of the O2 concentration decreased the H2O2 level rather than the in vitro developmental rate to the blastocyst stage.