Effects of prostaglandin F2alpha and nitric oxide on the secretory function of bovine luteal cells
2004
Korzekwa, A. (Institute of Animal Reproduction and Food Research, Olsztyn (Poland)) | Jaroszewski, J.J. | Bogacki, M. | Deptula, K.M. | Maslanka, T.S. | Acosta, T.J. | Okuda, K. | Skarzynski, D.J.
The objective of the present study was to investigate the influence of prostaglandin F2alpha (PGF2alpha) and nitric oxide (NO) on production of steroids and PGs by culturing bovine luteal cells obtained from ovaries on days 8-12 of the estrous cycle with a nitric oxide (NO) donor (Spermine NONOate), and a NO synthase inhibitor (NG-nitro-L-arginine methyl ester dihydrochloride: L-NAME). When the cells were exposed for 24 h to PGF2alpha (10E-7-10E-5 M), production of progesterone (P4) increased significantly at all doses used (P<0.05). Moreover, PGF2alpha stimulated PGF2alpha production (P<0.01), depressed testosterone (T) production (P<0.05), but did not affect synthesis of prostaglandin E2 (PGE2). Spermine NONOate decreased P4 production to 66%, 47% and 34% of the control concentration after treatment with 10E-5 M, 10E-4 M and 10E-3 M, respectively, but did not affect T production, and increased PGF2alpha synthesis (P<0.05) and PGE2 (P<0.01) at all doses used. L-NAME increased production of P4 (P<0.01) but did not affect (P>0.05) secretion of T, PGF2alpha and PGE2. Estradiol-17beta (E2) was detectable on the level of sensitivity of assay and was not significantly altered by any treatments. The overall results suggest that PGF2alpha and NO produced locally in bovine CL play roles in the regulation of the secretory function of the bovine CL as auto/paracrine factors.
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