Analysis of diffusible IAA by diode array detector
2004
Ohta, Y. (Niigata Univ. (Japan)) | Kojima, K. | Kikuchi, H. | Chino, S. | Niimi, Y.
Diffusible IAA obtained from bamboo shoot was analyzed in HPLC which connected a diode array (DA) detector, UV detector and fluorescent detector. At the UV detector, the peak which correspond to retention time (assumed peak) of IAA was detected, but assumed peak of IPA wasn't detected. At the fluorescence detector, assumed peaks of both IAA and IPA were detected. IAA quantity which was calculated from the assumed peak of IAA was about 25 times at 50% compared with 30% of methanol concentration of eluent. At the DA detector, the peak which overlapped with IAA assumed peak in case of 50% methanol was separated in 30% methanol. Maximum wavelength of spectrum which correspond to retention time (correspond spectrum) of IAA of 30% methanol and spectrum of IAA standard coincident. Further, in analyzed with the analysis software, too. purity of IAA assumed peak was 0.9902 and correlation of the IAA correspond spectrum and spectrum of standard was 0.9771. In conditions of this report, separation of IAA and impurity was shown with the DA detector.
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