Effects of serum deprivation on expression of proteolytic-related genes in chick myotube cultures
Nakashima, K. (National Inst. of Livestock and Grassland Science, Tsukuba, Ibaraki (Japan)) | Yamazaki, M. | Abe, H.
We previously reported that serum deprivation stimulates myofibrillar proteolysis in chick myotubes. In the present study, we examined the effect of serum deprivation on expression of the proteolytic-related genes (ubiquitin, proteasome, calpains, and cathepsin B) by real-time PCR of cDNA in chick myotubes. Myotubes were incubated with serum-free medium for 24h. Ubiquitin and proteasome subunits (C1 and C2) and calpains m-, micro-, and p94/calpain-3) but not cathepsin B mRNA expression were increased by serum deprivation. These results indicate that serum deprivation stimulates ubiquitin-proteasome and calpain proteolytic pathways, resulting in an increase in myofibrillar proteolysis in chick myotubes.
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