In vitro morphogenesis from pedicels of Hippeastrum x hybridum
2005
Huang, C.L. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) | Chang, K.C. | Okubo, H.
In vitro morphogenensis from pedicels of Hippeastrum x hybridum cv. Hermitage was investigated. Bulblet formation rates and number of bulblets were excellent when the concentrations of naphthyl acetic acid (NAA) and N6-benzyIadenine (BA) were the same. The combination of 5 mgl Sup(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) with 0.02-2 mgl Sup(-1) thidiazuron (TDZ) induced somatic embryogenesis with high rate and the combination of 5 mgl Sup(-1) 2,4-D and 2 mgl Sup(-1) TDZ gave the greatest number of somatic embryos among the treatments. High concentrations of TDZ (0.2-2mgl Sup(-1) promoted bulblet formation, but reduced root formation. Number of shoot primordia was high with higher 2,4-D) concentrations irrespective of TDZ. The highest number of bulblets (6.4) was obtained with 1 mgl Sup(-1) 2,4-D+0.2mgl Sup(-1) TDZ.
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