A storage method of tissues to keep cell viability for a short period before collecting carcass data
2004
Hayashi, N. (Gifu-ken. Livestock Research Inst., Kiyomi (Japan)) | Kato, S. | Sobajima, H. | Kobayashi, N. | Hirao, I. | Otani, T.
Our attempt is to enhance genetic improvement in Japanese Black Cattle using somatic cell nuclear transfer technology. For this purpose, it is to be desired that somatic cells obtained from cattle with carcass information on special, profitable traits are used as donor cells The present experiment was to investigate the relationship between storage condition of tissue samples for a short period and viability of cells. First, ear tissues were taken from 3 animals immediately after their slaughter The tissue samples were divided into five groups and each group was stored for 5 days at 4 deg C in one of five different ways : without using any medium and with immersing in one of four different storage media that were all based on phosphate buffer solution. Fresh tissues were used as control tissues. The tissue samples were used for cell culture to compare in fibroblast multiplication. The five types of storage treatments were effective in keeping good viability of cells with no detectable difference. Second, ear tissues taken from 140 animals immediately after their slaughter in a slaughter house were stored at 4 deg C for 2-4 days before collecting carcass data. Seven tissues selected randomly from them were used for cell culture, All of them gave good fibroblast multiplication and the obtained cells were found to tolerate passage culture and have a high viability even after freezing. These results suggest that storage of tissues at 4 deg C without using any storage medium is an easy method to keep cell viability for a short period before carcass data are collected.
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