Nutritional enrichment and cultivation of rotifers [Brachionus rotundiformis] by feeding of docosahexaenoic acid-enriched Chlorella vulgaris K-22
2004
Yukino, T. (Kagoshima Univ. (Japan)) | Hayashi, M. | Yoshimatsu, T. | Maruyama, I. | Murata, H.
The cultivation of rotifers using Docosa-Hexaenoic Acid (DHA)-enriched Chlorella vulgaris K-22 as a food was investigated. The exogenous DHA was incorporated into neutral lipids in DHA-enriched C. vulgaris cells. The highest percentage of DHA in the total fatty acids of cellular lipids reached 82.9%. During the nutritional enrichment of rotifers with DHA-enriched C. vulgaris, the amount of DHA in the rotifers rapidly increased to 3.3% over 6 hours. During the course of the 3-day cultivation of the rotifers, those provided with DHA-enriched C. vulgaris showed higher population growth, and maintained higher stable levels of DHA, than rotifers that were given non-enriched C. vulgaris. Thus, cultivation by providing rotifers with DHA-enriched C. vulgaris resulted in rotifers possessing high DHA content without the use of additional nutritional enrichment procedures.
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