Comparative study on saikosaponin contents in root of unsexual propagation and sexual reproduction in Bupleurum falcatum
2005
Shon, T.K.(Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) | Park, S.K. | Furuya, T. | Lee, S.C.
Production of major constituents in medicinal plants mainly depends on their origin; hence it is possible to produce more of such constituents through selection of potential cultivars. Differences in saikosaponin content and agronomic characteristics between the roots of regenerated plantlets through anther culture and plats cultivated by seed in Bupleurum falcatum were examined. There were significant differences between the means of regenerated plantlet and plant cultivated in fresh weight of aerial part, dry weight of aerial part and dry root length. The saikosaponin contents differed between the different origins. The content of saikosaponin a in plants with chromosome number 2n=20, originated from Korea, was higher than that of 2n=26, from Japan, in both regenerated plantlets through anther culture and plants cultivated by seed propagation. But, total saikosaponin contents determined by HPLC were similar between the roots of regenerated plantlets and cultivated plants. Unsexual propagated plants through anther culture have proven to be more homogeneous than those of sexually reproductive plants by seed. To obtain uniform characteristics in B. falcatum, the unsexual propagation through anther culture is one of the effective methods for stable and uniform production in B. falcatum.
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