Effect of continuous and interval administration of peptidoglycan on innate immune response and disease resistance in Japanese flounder Paralichthys olivaceus
2006
Galindo-Villegas, J.(Ehime Univ., Matsuyama (Japan)) | Masumoto, T. | Hosokawa, H.
Peptidoglycan (PG), derived from Brevibacterium lactofermentum, is known to enhance innate immune response and disease resistance. The present study investigated the effect of the interval dietary administration of PG on the innate immune response and the disease resistance against Edwardsiella tarda in Japanese flounder. Continuous and interval dietary administration at 2.5% body weight per day of supplemental 0.3% PG in dry diet were tested following one of four patterns for 40 days: control group (only control diet) , 10/10 group (PG diet/control diet, every 10 days) , 15/15 group (PG diet/control diet, every 15 days) or daily group (only PG diet) . Innate immune response (serum lysozyme activity, chemotaxis and intracellular respiratory burst) were evaluated after 7, 10, 15, 20, 25, 30 and 40 days. Furthermore, three bacteria challenges were conducted on days 10, 25 and 40. Significant enhancements in serum and leucocyles activities were observed along the trial. Chemotaxis was enhanced in all sampling day in most PG-administrated groups. The enhanced disease resistance was positively correlated with the enhancement of innate immune response in au PG-treated groups after 10 days and only in both 10/10 and 15/15 groups on days 25 and 40. These findings suggest that interval administration of PG persist the enhanced disease resistance of Japanese flounder against E. tarda infection through cyclical activation of the immune system, while daily administration for more than 15 days has inert effect in disease resistance.
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