Lipase-Catalyzed Synthesis of Structured Lipids with Capric and Conjugated Linoleic Acid in a Stirred-Batch Type Reactor
Shin, J.A.;Lee, K.T.(Chungnam National University, Daejeon, Republic of Korea)E-mail:[email protected]
Structured lipid (SL) was produced from soybean oil with molar ratio of 1：2：2 (soybean oil：capric acid：CLA) using Chirazyme∨ⓡ L-2 lipase (4％ by weight of total substrates). The reaction was conducted for 24 hr at 55℃ in a 1 L stirred-batch type reactor. SL-soybean oil contained 4.9 mol% capric acid and 4.1 mol% CLA, respectively. Iodine value of SL-soybean oil was reduced than that of soybean oil due to the incorporated capric acids. Tocopherol content in SL-soybean oil was 18.2 mg/l00 g. SL-soybean oil appeared more yellowish color than soybean oil. Reverse-phase HPLC showed that SL-triacylglycerol species containing capric acid consisted of about 12.6 area%.
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