Characterization of the Catabolite Control Protein (CcpA) Gene from Leuconostoc mesenteroides SY1
2005
Park, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Park, J.S. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea) | Jeong, S.J. (Gyeongsang National University, Jinju, Republic of Korea) | Chun, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, J.H. (Kyonggi University, Suwon, Republic of Korea) | Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
The ccpA gene encoding catabolite control protein A (CcpA) of Leuconostoc mesenteroides SY1, a strain isolated from kimchi, was cloned, sequenced, analyzed for transcript, and overexpressed in Escherichia coli. The ccpA ORF (open reading frame) is 1,011 by in size, which can encode a protein of 336 amino acid residues with a molecular mass of 36,739 Da. The transcription start site was mapped at a position 49 nucleotides upstream of the start codon, and promoter sequences were also identified. The putative cre site overlapped with the -35 promoter sequence.
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