Comparison of Phylogenetic Characteristics of Viable but Non-Culturable (VBNC) 16S Bacterial Populations in the Pine and Quercus Forest Soil by 16S rDNA-ARDRA
2006
Han, S.I. (Mokwon University, Daejeon, Republic of Korea) | Kim, Y.J. (Mokwon University, Daejeon, Republic of Korea) | Whang, K.S. (Mokwon University, Daejeon, Republic of Korea), E-mail: [email protected]
In this study was performed to analyze quantitatively the number of viable but non-culturable bacteria in the Pine and Quercus forest soil by improved direct viable count (DVC) and plate count (PC) methods. The number of living bacteria of Pine and Quercus forest soil by PC method were less then 1% of DVC method. This result showed that viable but non-culturable (VBNC) bacteria existed in the forest soil with high percentage. Diversity and structure of VBNC bacterial populations in forest soil were analyzed by direct extracting of DNA and 16S rDNA-ARDRA from Pine and Quercus forest soil.
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