Direct Somatic Embryogenesis and Plant Regeneration in Cineraria (Senecio cruentus)
2005
Nam, E.Y. (Rural Development Administration, Suwon, Republic of Korea) | Kim, G.H. (Gyeongsang National University, Jinju, Republic of Korea) | Jeong, B.R. (Gyeongsang National University, Jinju, Republic of Korea)
This study was conducted to investigate the conditions involved in efficient somatic embryogenesis and plant regeneration of cineraria 'Jester Pink'. Explants were taken from cotyledons and hypocotyls. Initiation and development of somatic embryos occurred through a two-step process. Explants were first induced to globular embryos on the MS medium supplemented with several plant growth regulators (4.5-13.5 μM 2,4-dichlorophenoxy acetic acid and 1.5-4.5 μM benzylademine, kinetin, or thidiazuron). Somatic embryos formed directly on the surface of explants without the callus phase after 5 weeks of culture. In the next step, somatic embryos were transferred to the plant growth regulator-free or the medium supplemented with 3.8-11.4 μM abscisic acid, 0.1-0.3% (w/v) polyethylene glycol and 4-6% (w/v) sucrose for embryo maturation.
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