Adventitious Bud Induction and Plant Regeneration from Cotyledon Explants of Camellia japonica L.
2005
Kim, K.S. (Dongshin University, Naju, Republic of Korea) | Hwang, S.J. (Dongshin University, Naju, Republic of Korea), E-mail: [email protected] | Pyo, B.S. (Dongshin University, Naju, Republic of Korea) | Kim, S.M. (Dongshin University, Naju, Republic of Korea)
Culture conditions for plant regeneration of Camellia japonica were achieved by organogenesis in explants of cotyledon. Seed cotyledons were cultured on MS medium containing various auxin, 2,4-D or NAA and cytokinins BA. The adventitious shoot buds were efficiently formed without embryogenesis on the basal region og cotyledon cultured on MS medium supplemented with 0.1 mg/ℓ 2.4-D and 1 mg/ℓ BA. Seed cotyledons could be used as a source of explants in experiments of genetic transformation of the genotypes evaluated for improving the efficiency of production of transgenic Camellia plants.
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