The effect of fungicides on the microflora of grains
Loiveke, H.(Estonian Research Inst. of Agriculture, Harjumaa (Estonia))E-mail:[email protected]
This study was carried out in order to investigate the effect of fungicides on the microflora of grain yield (the winter wheat variety 'Sani') in the field trials of 2002-2003. Variants on background N0 and N150: 1. Untreated, 2. Bumper Super (a.i. propiconazole + prochloraz) 1.0 l haE-1, 3. Tilt 250 EC (a.i. propiconazole) + Sportak 45 EC (a.i. prochloraz) (0.36 + 0.89 l haE-1), 4. Tilt 250 EC (a.i. propiconazole) 0.5 l haE-1. Application for the control of Leptosphaeria nodorum E. Mueller was made on 23.05.2002 on GS 39 and on 17.06.2003 on GS 47-49. The date of harvest was 25.07. in 2002 and 11.08. in 2003. The total number of aerobic mesophilic bacteria was estimated on the PCA (Plate Count agar = Casein-peptone-glycose-yeast agar, ICC Standard No. 125, 1978), whereas for fungi and yeasts, RBCA (Rose bengal chloramphenicol agar, ICC Standard No. 146, 1992) was used. The species and number of Fusarium Link. ex Fr. were defined on Nash and Snyder selective medium. The classification of Fusarium isolates was made according to Gerlach and Nirenberg (1982). The toxicity of Fusarium isolates was tested on the basis of the size of the growth inhibition zone of Bacillus stearothermophilus (Watson, Lindsay, 1982).
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