In vitro Culture of Adventitious Roots from Dioscorea nipponica Makino for the Production of Steroidal Saponins
2005
An, J.H. (Andong National University, Andong, Republic of Korea) | Son, K.H. (Andong National University, Andong, Republic of Korea) | Sohn, H.Y. (Andong National University, Andong, Republic of Korea) | Kwon, S.T. (Andong National University, Andong, Republic of Korea), E-mail: [email protected]
Effects of growth regulators on growth of adventitious roots and accumulation of steroidal saponins, such as dioscin, prosapogenin A and prosapogenin C, in cultures of Dioscorea nipponica were determined. The maximum growth of adventitious roots was observed in MS medium supplemented with 30 g/L sucrose and 1.0 mg/L NAA. Addition of BA in combination with NAA appeared to be no effective in the growth of adventitious roots. Among the twenty different adventitious roots formed from different seeds, strain No. 10 was selected based on production ability of dioscin, and its stability through the successive liquid culture.
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