Regulation of GFP Expression Using the Tetracycline Inducible Retroviral Vector System
2005
Koo, B.C. (Catholic University of Daegu, Gyungsan, Republic of Korea) | Kwon, M.S. (Catholic University of Daegu, Gyungsan, Republic of Korea) | Kim, T.A. (Catholic University of Daegu, Gyungsan, Republic of Korea), E-mail: [email protected]
One of the critical problems to be solved in transgenic animal production is uncontrollable constitutive expression of foreign genes, which usually results in serious physiological disturbances in the transgenic animal. To circumvent this problem, we constructed and tested two retrovirus vectors designed to express the GFP(green fluorescent protein) gene under the control of the tetracycline-inducible promoters. To maximize the GFP gene expression at turn-on state, WPRE(woodchuck hepatitis virus posttranscriptional regulatory element) sequence was introduced into the retrovirus vectors at downstream region of either the GFP gene or the sequence encoding rtTA(reverse tetracycline-controlled transactivator).
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