Effects of Genotypes on In Vitro Maturation and Fertilization of Frozen-Thawed Porcine Oocytes
2005
Jia, Y.H. (Kangwon National University, Chuncheon, Republic of Korea) | Jin, H.J. (National Livestock Research Institute, RDA, Namwon, Republic of Korea) | Wee, M.S. (Kangwon National University, Chuncheon, Republic of Korea) | Cheong, H.T. (Kangwon National University, Chuncheon, Republic of Korea) | Yang, B.K. (Kangwon National University, Chuncheon, Republic of Korea) | Park, C.K. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: [email protected]
In the present study, we investigated the effects of genotypes on in vitro maturation and fertilization in porcine fresh/frozen-thawed oocytes. The porcine cumulus-oocyte complexes (COCs) were divided into four groups according to whether they were: (1) in vitro matured; (2) cryopreserved and in vitro matured; (3) in vitro fertilized and (4) cryopreserved, and in vitro fertilized. Maturation of porcine COCs was accomplished by incubation in NCSU23 medium. Immature oocytes were cryopreserved by Open Pulled Straws (OPS) method according to Vajta et al., (1998). Oocytes stained by Acetic-Orcein method were observed under the microscope.
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