Enzymatic synthesis of oligosaccharides and alkylglycosides in water-organic media via transglycosylation of lactose
2006
Bankova, E. | Bakalova, N. | Petrova, S. | Kolev, D.
The synthesis of oligosaccharides and alkylglycosides via transglycosylation reaction of lactose by Aspergillus oryzae beta-glycosidase was studied in the presence of water miscible organic solvents- DMSO (N',N'-dimethylsulfoxid) and DMF (N',N'- dimethylformamide) and water immiscible hydrophilic alcohols - isopropanol, secondary butanol and isobutanol. In the presence of DMSO and DMF, both the transglycosylation and hydrolytic activities of the enzyme were suppressed. The yield of trisaccharides after 24h incubation was about 5% in the presence of 20% DMSO or 20% DMF. The highest transglycosilation activity of Aspergillus oryzae beta-glycosidase was demonstrated in the presense of isobutanol.
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Эту запись предоставил Institute of Agricultural Economics