Determination of ferulic acid in Angelica sinensis root by PC-spectrophotometry method
2005
Gong Minggui，Dong Juan’e，Li Jintong
Китайский. 直接用紫外分光光度法测定当归提取液中阿魏酸含量时，由于杂质干扰较大，影响测定精度，而采用纸层析分离可以将杂质除去。研究对纸层析的展开系统、不同萃取溶剂的稳定性等因素进行比较分析后，建立了纸层析分离-紫外分光光度法测定当归中阿魏酸含量的方法。纸层析分离条件为：以氯仿、甲醇、水和冰醋酸按体积比2∶2∶6∶0.5配比作为展开剂，检测波长为330 nm，加样回收率为99.61%，标准差为2.79%，吸光度在0.020～0.061时线性关系良好。
Показать больше [+] Меньше [-]Английский. Determining precision was affected by the impurities in the extract of Angelica sinensis when the content of ferulic acid was determined directly by UV-spectrophotometry. Paper chromatography (PC) was used to eliminate these impurities. The separation condition of PC and the stability of different extraction solvents were studied. Based on the results, a determination method of ferulic acid in Angelica sinensis by PC-spectrophotometry was established. The elution system for PC was: chloroform∶methanol∶water∶acetic acid (6∶2∶2∶0.5), the wavelength for spectrophotometry was 330 nm. The recovery rate of the method was 99.61% and relative standard deviation (RSD) was 2.79%.The absorbency shows good linearity relation between 0.020 and 0.061.
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