Identification, cloning, and expression of the Scytalidium acidophilum XYL1 gene encoding for an acidophilic xylanase
Balaa, B.A.(University of Namur (Belgium)) | Wouters, J. | Dogne, S. | Rossini, C. | Schaus, J.M. | Depiereux, E. | Vandenhaute, J. | Housen, I.
We cloned XYL1, a Scytalldium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYLlpalphaA protein was expressed in Pichia pastoris using the pPICZalphaA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYLlp showed an optimum activity at pH 3.2 and 56 deg C. The Michaelis-Menten constants were determined.
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Библиографическая информация
Нумерация страниц
pp. 269-272
Другие темы
Clonage moleculaire; Clonacion molecular; Expression des genes; Ingenieria genetica; Expresion genica; Microorganismos xilanoliticos; Genie genetique
Язык
Английский
Примечание
Summary (En)
2 fig. 22 ref.
Тип
Summary
2007-05-15
AGRIS AP
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