Isolation and characterization of a bluegill-degrading microorganism, and analysis of the root hair-promoting effect of the degraded products
Sanpa, S.(Ritsumeikan Univ., Kusatsu, Shiga (Japan). Faculty of Science and Engineering) | Sumiyoshi, S. | Kujira, T. | Matsumiya, Y. | Kubo, M.
Bluegill-degrading bacteria were isolated from various environmental sources. Brevibacillus sp. BGMl degraded bluegil1 efficiently at 50 deg C, and its culture supernatant showed the highest peptide and amino acid concentrations as trichloroacetic acid (TCA) soluble fraction (ASF) (10.7 mg/ml) of all supernatants obtained with bluegill as a substrate. Strain BGM1 secreted a protease(s) into the medium, and the concentration of peptides and amino acids gradually increased. The fertile effect of the degraded bluegill products (DGP) on Brassica rapa was also investigated. The root hair density of B. rapa grown with DGP a, a concentration of 30 microg peptides and amino acids/ml was about 1.7 times higher than when grown with the same concentration of undegraded bluegill. DGP was shown to increase root hair numbers and adventitious root formation. The results of this study suggest that a specific peptide(s) for promotion of root hair is produced from the order Perciformes with a protease(s) from BGM1.
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