An in Vitro Vitellogenin Bioassay for Estrogenic Substances in the Rhynchocypris Oxycephalus
2006
Shin, J.A. (Kyonggi University, Suwon, Republic of Korea) | Song, J.Y. (Kyonggi University, Suwon, Republic of Korea) | Lim, H.Y. (Kyonggi University, Suwon, Republic of Korea) | Chang, M.H. (Kyonggi University, Suwon, Republic of Korea) | Lee, H.M. (Kyonggi University, Suwon, Republic of Korea) | Chung, K.H. (Kyonggi University, Suwon, Republic of Korea) | Lim, Y.K. (Cheju National University, Jeju, Republic of Korea) | Yoon, B.S. (Kyonggi University, Suwon, Republic of Korea), E-mail: bsyoon@kyonggi.ac.kr
Vitellogenin (Vtg), a phospholipoglycoprotein precursor of egg yolk is synthesized and secreted from the liver in response to estrogens in femals fish. Vtg is normally undetectable in the blood of male fish, but can be induced by exposure to chemicals possessing estrogenic activity. Thus, the presence of Vtg in blood of male fish can serve as a useful biomarker for assessing previous exposure to estrogenic compounds. In the present study, Vtg was abnormally expressed in Rhynchocypris oxycephalus using estradiol benzoate (E₂).
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