Increased Ethylene and Decreased Phenolic Compounds Stimulate Somatic Embryo Regeneration in Leaf Thin Section Cultures of Doritaenopsis Hybrid
2006
Park, S.Y. (Korea Forest Research Institute, Suwon, Republic of Korea) | Shin, K.S. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea) | Paek, K.Y. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [email protected]
The effects of endogenous levels of ethylene and phenolic compounds on somatic embryogenesis, medium-browning, and peroxidase activity were evaluated in thin section cultures of Doritaenopsis. Cultures were maintained for 8 weeks with four different treatments: ⅰ) thick leaf segment culture, ⅱ) thin leaf section culture, ⅲ) thin leaf section culture with ventilation, or ⅳ) thin leaf section culture after explants were first washed. Explants cultured in closed vessels produced a larger number of somatic embryos than those reared in the ventilated vessels.
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