Identification of Rhizoctonia solani isolates from sugar beet roots by analysing the ITS region of ribosomal DNA
2007
Stojsin, V.,Faculty of Agriculture, Novi Sad (Serbia). Department for Environment and Plant Protection | Budakov, D.,Faculty of Agriculture, Novi Sad (Serbia). Department for Environment and Plant Protection | Jacobsen, B.,Montana State University, Bozeman, 59715 MT (USA ). Department of Plant Science and Plant Pathology | Grimme, E.,Montana State University, Bozeman, 59715 MT (USA). Department of Plant Science and Plant Pathology | Bagi, F.,Faculty of Agriculture, Novi Sad (Serbia). Department for Environment and Plant Protection | Jasnic, S.,Faculty of Agriculture, Novi Sad (Serbia). Department for Environment and Plant Protection
Four isolates of Rhizoctonia solani from sugar beet roots showing characteristic crown and root rot symptoms, collected from different localities in the Vojvodina Province (Serbia), were chosen and compared to the well-characterized Rhizoctonia solani isolate R9, AG 2-2 IV, from the USA. All Vojvodinian isolates showed medium level of pathogenicity and were able to cause crown and root rot symptoms on inoculated sugar beet roots. Based on anastomosis reaction, isolates from Vojvodina did not belong to the AG 2-2 group. Sequencing of the ITS (internal transcribed spacer) region of ribosomal DNA was perfomed on the Vojvodinian isolates from R9 in order to determine their relatedness. Sequence analysis showed that these isolates were different than R9 and were closely related (99-100% sequence homology) to anastomosis group 4, subgroup HG II.
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