Isolation, qualitation and quantitation of substances in herbal drugs by monoclonal antibody
2004
Penpun Wetwitayaklung(Silpakorn University. Sanam Chandra Palace Campus, Nakhon Pathom (Thailand). Faculty of Pharmacy. Dept. of Pharmacognosy)
Immunoaffinity column and immunoassay were developed for identifying and analyzing active substances in herbal medicines beyond conventional methods such as TLC, CC, HPLC and GC. Both methods use IgGs or monoclonal antibodies of selected separating and analyzing substances as probes. They are sensitive and specific methods to the substances, being superior to conventional methods. Immunoaffinity column could isolate and purify in single step separation. Immunoassay could analyse substances in picro-nanogram concentration. However, cross reactivity of these methods might occur within similar structure substances. Immunoaffinity column and immunoassay are suitable for detecting substances which have sugar moiety in their structures, i.e., saponin, glycoside, glucuronide and aminosugar. They are convenient for analyzing those substances in the combination of herbal drug preparations.
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Эту запись предоставил Kasetsart University