[Application of RAPD-markers in the introgressive selection of potato at the diploid level]
2007
Voronkova, E.V. | Lisovskaya, V.M. | Golenchenko, S.G. | Makhan'ko, O.V. | Luksha, V.I. | Ermishin, A.P., National Academy of Sciences (Belarus). Institute of Genetics and Cytology
Research results of application of RAPD analysis for the investigation of introgression of the specific loci of allotetraploid (S. acaule, S. stoloniferum, S. polytrichon) and Mexican 1EBN diploid species S. pinnatisectum and S. bulbocastanum into hybrid material of cultured potato (Solanum tuberosum, L.) were presented. The RAPD primer-set with polymorphism level which made it possible to realize the successful search for the specific loci of potato wild species and their further revelation within the interspecies hybrids was selected. Almost all selected hybrid material had the certain amount of the genetic material of wild donors. The specific loci of wild species remained after the backcrossing of selected diploids. Introgression level of both diploid and triploid hybrids was rather higher among the stock obtained on the basis of S. stoloniferum (35,76% and 62,37% respectively) and especially on the basis of S. polytrichon (69,14% and 77,77% respectively). In S. acaule stock the same indices were 22,57% for diploids and 58,91% for totraploids that proved the impossibility of some valuable genes transfer from S. acaule in comparison with Longipedicillata species. Evaluation of the introgression level of the specific loci of wild species of interspecies hybrids revealed a high percentage of wild species genome preservation in tetraploid and somatic hybrids. Diploid hybrids on the basis allotetraploids and diploids on basis of somatic hybrids preserved nearly the quarter of wild species material. The analysed level of introgression of the specific loci proved the preservation of a large proportion of valuable for selection genes of allotetraploid and 1EBN donors
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