The determination of avidin in genetically modified maize by voltammetric techniques
Petrlova, J.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Chemie a Biochemie | Krizkova, S.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Chemie a Biochemie | Supalkova, V.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Chemie a Biochemie | Masarik, M.,Akademie Ved, Brno (Czech Republic). Biofyzikalni Ustav | Adam, V.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Chemie a Biochemie | Havel, L.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Biologie Rostlin | Kramer, K.J.,US Dept. of Agriculture, Manhattan (USA). Grain Marketing and Production Research Center | Kizek, R.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Chemie a Biochemie
Plant species such as barley, rice, sunflower, wheat and maize are very common objects of interest of genetic modification (GMO); therefore, the development of specific assays for their detection and quantification are needed. Furthermore, the production and trade of genetically modified lines from an increasing number of plant species brings about the need for control within research, environmental risk assessment, labeling-legal, and consumers' information purposes. Electrochemical sensors and biosensors based on modification of working electrode could be suitable tools for these purposes. Here, we report using an avidin-modified carbon paste electrode for rapid and sensitive determination of avidin in plant extract solution and in a transgenic maize extract. The process could be used to determine avidin concentrations up to 3pM in solution and 170nM in a maize seed extract. Moreover, we applied the method to analyze different maize flours.
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