Plantlet regeneration from fascicular buds of seedling shoot apices of Pinus roxburghii Sarg
Kalia, R.K.,Forest Research Inst., Dehradun (India). Tissue Culture Lab. | Arya, S.,Forest Research Inst., Dehradun (India). Tissue Culture Lab. | Kalia, S.,Forest Research Inst., Dehradun (India). Tissue Culture Lab. | Arya, I.D.,Forest Research Inst., Dehradun (India). Tissue Culture Lab.
Shoot apices of Pinus roxburghii Sarg were cultured on Murashige and Skoog's medium (MS) supplemented with cytokinins [6-benzyladenine (BA), kinetin and N-benzyl-9-(2-tetrahydropyranyl) adenine (BPA)] alone and in combination with alpha-napthaleneacetic acid (NAA). Of the three cytokinins tested at varying concentrations, medium supplemented with 10 microM BA was found optimal in respect of explant responsiveness (97.22%) and average number of buds induced per explant (7.42). The concentration of cytokinins in the induction medium had a profound effect on rate of elongation of induced buds on MS basal medium containing 0.5% activated charcoal. Further, shoots induced on lower concentrations of BA increased up to 2.4 times in length in 4 weeks. Decapitation of the explant enhanced the rate of axillary bud elongation. Proliferating shoot cultures were established by sub-culturing the axillary shoots on MS supplemented with 10 microM BA. Shoots 2-3 cm in length were suitable for culturing as more buds were induced on them than on longer or shorter shoots. Root primordia were induced on 70.83% shoots when transferred to 1/2 MS medium supplemented with 5.0 microM NAA. Elongation of root primordia (60%) was achieved in liquid 1/2 MS basal medium. The plantlets were successfully transferred to soil after hardening; the time period from initiation of shoot buds to transplantation being 20-22 weeks.
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