Histological and ultrasonographic monitoring of folliculogenesis in puerperal ewes after spring lambing
2008
Vlckova, R.,Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic). Katedra Anatomie, Histologie a Fyziologie | Valocky, I.,Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic). Klinika Porodnictva, Gynekologie a Andrologie | Lazar, G.,Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic). Klinika Porodnictva, Gynekologie a Andrologie | Sopkova, D.,Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic). Katedra Anatomie, Histologie a Fyziologie | Maracek, I.,Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic). Katedra Anatomie, Histologie a Fyziologie
The objective of the present study was to analyse the follicle status after parturition in non-lactating Improved Valachian ewes. Laparotomy with following ovariectomy was performed after lamb weaning (spring) on days 17, 24 and 32 after parturition. The ovaries were USG analysed with 5.0 MHz linear and 7.5 MHz convex transducer. Follicular data were analysed quantitatively and qualitatively. The ovaries were collected at the end of laparotomy and fixed in 10% formalin. The sections of the ovarian tissue were stained with haematoxylin and eosin and Azan staining. Ovarian slides were microscopically studied and analysed by LUCIA-G ver. 4.71. The differences in the ovary size were not significant. The number of follicles less than 3 mm monitored by USG on day 32 was higher than that on day 17 after parturition. The image analysis of the ovary sections showed significantly higher numbers in the total follicles and the follicles over 3 mm. The rate of atresia was 82% and 89% on day 17 and on day 32 post partum, respectively. We observed single ovulation on day 17 and double ovulations on days 24 and 32 post partum. More than half of the total number of antral follicles visible on the ovary surface was prepared for recruitment and this number was higher on day 32 post partum. Follicle selection connected with a relatively low rate of atresia and responsible preovulatory follicle sizes opened the way for selecting more follicles for the dominance process and thus also for the occurrence of double ovulations.
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