Improvement of in vitro androgenesis in niger using amino acids and polyamines
Hema, B.P.,Karnatak Univ., Dharwad (India). Plant Tissue Culture Lab. | Murthy, H.N.,Karnatak Univ., Dharwad (India). Plant Tissue Culture Lab.
The effects of amino acids (arginine, aspargine, cysteine, glutamine, glycine and proline) and polyamines (putrescine and spermidine) on embryogenesis and plant regeneration from cultured anthers of Guizotia abyssinica cv. Ootacamund was studied. Supplementation of amino acids (0.5-5.0 mM) to the induction medium (individually or in combination) improved embryo yield. B5 medium supplemented with 2 mM proline, 10 microM 2,4-dichlorophenoxyacetic acid, 2 microM kinetin and 0.2 M sucrose induced the highest number of embryos (63 per 60 anthers cultured). Addition of polyamines (5-200 microM) to the same medium also enhanced the rate of embryogenesis.
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