Study Of Callus Induction And Plant Regeneration Of Maize Genotypes (Zea Mays L.) Using Immature Embryo Culture
2007
Khavari Khorasani, S., Assistant Professor and Researcher of Agricultural and Natural Resources of Khorasan Razavi Province Center, | Mansouri, S., M.Sc. Student of Plant Breeding at Tarbiat Modares University. | Moieni, N., Assistant Prof., of Plant breeding at Tarbiat Modares University, | Mousavi, A., Assistant Professor and Researcher of national Institute for Genetic Engineering and Biotechnology.
In this study, response of 10 maize genotypes consisted of inbred lines, single and three-way crosses with inbred line A188 as control to callus induction and plant regeneration was evaluated at Tarbiat Modarres University in 2004. Immature embryos were excised from donor plants 10-14 days after selfing and were culturd on N6 medium. The non-embryogenic, rizhogenic and watery calli were removed and the embryogenic calli (type 1 and type 2 ) were subcultured on regeneration medium. Then the normal plantlets were transferred to sterilized soil. The results showed significant differences among corn genotypes for producing embryogenic callus. The highest frequency of embryogenic callus produced by S61, A188, SC709, with 92%, 88% and 82%, respectively. Analysis of variance for regeneration on 3 genotypes (S61, A188 and SC709) showed significant differences between genotypes. Hybrid SC709 with 36.12% and inbred line S61 with 14.25% had the highest and lowest percent of plant regeneration, respectively.
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