Mass Production Of Common Yew (Taxus Baccata) Plantlets By Invitro Embryo Culture
2006
Nikvash, N., MSc. Student, Payame. Nour University, Tehran, Iran. | Asareh, M.H., Research Institute of Forests and Rangelands, | Ghorbanli, M., Scientific Board Member of Gorgan Azad University Gorgan, Iran. | Ghamari Zare, A., Research Institute of Forests and Rangelands.
Taxus baccata is an endangered forest tree species which its regeneration is very low. Embryo culture carried out to find a practical method for seedling mass propagation. Seeds collected in December 2004 from its natural stand at Zarrin gol valley of Ali Abad Katol in Golestan province, Iran. The seeds were immersed under running water four treatment used to sterilize the seeds. The best treatment (a=%1) was Benomyl 1%.ethanol alcahol 70%, Sedium hypochlorite 3% and mercuric chloride 1%. Sixty seven percent of the treated seeds were uncontaminated from bacteria and fungi. Bassd on the method experienced in this research embryos separated from the seeds walls and endosperm. As a result many embryos were tested under inviro method at short period. The embryos were cultured on Ms Containing casein, yeast, ascorbic acid and activated charcoal (0.1, 1.0, 0.1 and 5.0 gr-1 respeectively). Plantlets were recultured every two weeks in order to increase the shoot height to 5cm. Monthly growth rate of plantlets was 0.7cm. The plantlets were transplanted to jiffy plate after few reculturing then watered at two first weeks with liquid Ms nutrition solution containing antibiotic (Carbonicilin). In order to increase root development, the young plantlets growing in jiffy Plate wer treated with IBN and NAA (1.0 and 0.5mg-1 respectively) after two weeks and enough growth (shoot and root) plantlets wer translanted in to soil and kept in a growth room for three months at 25آ°c for 14 hours in light and 17آ°c for 8 hours in dark. All seedling had normal growth.
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