Histopathologic and EM finding of cutaneous leishmaniasis in reservoir hosts in Fars Province and differentiation of genomic differences by PCR method.
2006
Paykari, Hbib Ol- Lah | Oryan, Ahmad | Mehrbani, Davud | Mo`tazediyan, Mohammad Hossein | Mansuri | Hatam, Gholam Reza
Cutaneous leishmaniasis (CL) with diverse clinical manifestations is prevalent and remains a major public health problem in Iran and in Southern Iran, the incidence has been doubled over the last decade. The present study is on the potential role of rodents in the epidemiology of CL in Kharameh District in Shiraz, Southern Iran. From April 2004 to April 2005, a total of sixteen rodents were collected in live traps from endemic area of CL in Kharameh district in Shiraz, Southern Iran. Using Evans medium for culture, specific polymerase chain reaction and isoenzyme electrophoresis methods were performed to characterize the parasite and the parasite morphology was studied. The rodent species was Tatera indica. Three samples from Tatera indica were found positive (2 males and 1 female in Kafdehak and Sejel-Abad villages) and identified as L. major. Macrophages in the bone marrow of femaoral bone were infected to amastigote form of the parasite. It seems that T. indica is the reservoir host for CL in Kharameh, district in Shiraz, Southern Iran. It was shown that bone marrow of rodents to be the tissue of choice for light and electron microscopy of Leishmania.
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