Comparative Assessment Of Electrophoretic Patterns Of Fasciola Somatic Antigens In Different Hosts
Meshgi, B., Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran. | Eslami, A., Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran | Hossieni, S.H., Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran | Hemmatzadeh, F., Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran | Hooshmand, E., Faculty of Agriculture, Eslamic Azad University, Rasht, Iran.
To determine the electrophoretic patterns of Fasciola hepatita and F. gigantica, the former was collected from sheep and buffalo infected liver and the latter from that of cattle and camel and were washed in PBS. Somatic antigens of both species were prepared though homogenization and centrifugation of adult flukes. To show the eclrophoretic patterns of somatic antigens, the prepared antigens were electrophoresed using SDS-PAGE. Our findings revealed that proteins of 18-70 kDa molecular weights were present in both species in different hosts. Although a 57 kDa protein was seen in Fasciola gigantica of cattle and camel only. Meanwhile two bands of 54 and 68 kDa were seen exclusively in F.hepatica of sheep and buffalo. A band of 64 kDa was shown in F.hepatica of buffalo. According to our results in any study on immunological or immunodiagnostic of Fasciola spp, the species trematode as well as their hosts should be taken in consideration.
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