Identification of phytoplasmas in vectors and potential vectors of grapevine yellows in Marches region, Central-Eastern Italy [Vitis vinifera L.]
2006
Riolo, P. | Murolo, S. | Riga, F. | Nardi, S. | Isidoro, N. | Romanazzi, G.
Английский. Bois Noir (BN) and Flavescence Dorée (FD) are grapevine yellows able to induce severe losses on grapevine in Italy. BN and its vector, Hyalesthes obsoletus, are widely spread in Marches region, while FD, transmitted by Scaphoideus titanus, was found in 2 vineyards, in absence of its vector. Investigations were carried out to identify phytoplasmas associated with vectors and potential vectors of grapevine yellows found in vineyard ecosystems of the region. Total DNA from adults of H. obsoletus, Anaceratagallia laevis, A. ribauti, Euscelis lineolatus, Laodelphax striatellus, Neoaliturus fenestratus and Psammotettix alienus was extracted and analysed by PCR and RFLP. Candidatus Phytoplasma solani, causal agent of BN, was found in H. obsoletus and N. fenestratus. Clover phyllody phytoplasma, belonging to the 16Sr- IC subgroup, was found in E. lineolatus, in almost all insects associated with Candidatus Phytoplasma solani
Показать больше [+] Меньше [-]итальянский. Il Legno Nero (LN) e la Flavescenza Dorata (FD) sono i giallumi della vite in grado di indurre le maggiori perdite economiche in Italia. La ricerca ha avuto come obiettivo l'identificazione dei fitoplasmi associati a vettori e potenziali vettori di giallumi presenti nell'agroecosistema vigneto della regione. Adulti di Hyalesthes obsoletus, Anaceratagallia laevis, A. ribauti, Euscelis lineolatus, Laodelphax striatellus, Neoaliturus fenestratus e Psammotettix alienus sono stati sottoposti ad estrazione del DNA totale, analizzato mediante nested-PCR ed RFLP. Le indagini hanno evidenziato la presenza di Candidatus Phytoplasma solani, agente di LN, in H. obsoletus e N. fenestratus. Il Fitoplasma della fillodia del trifoglio, appartenente al sottogruppo 16Sr-IC, è stato rinvenuto in E. lineolatus, quasi sempre associato a Ca. Phytoplasma solani
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